https://has.hcu.ac.th/xmlui/handle/123456789/8 คณะวิทยาศาสตร์และเทคโนโลยี /Faculty of Science and Technology Sat, 12 Sep 2026 14:37:13 GMT 2026-09-12T14:37:13Z https://localhost:443/jspui/retrieve/3f474818-1b48-4aef-a685-1aac22f75eb3/Faculty Logo_Science and Technology.jpg https://has.hcu.ac.th/xmlui/handle/123456789/8 https://has.hcu.ac.th/xmlui/handle/123456789/6216 Title: Production and characterization of a novel high-alkaline and thermal stable lipase from Bacillus sp. HCU3-2 and potential application as detergent formulation Authors: Piyaporn Supakdamrongkul; Aunchalee Wongthong; Suparud Komkaew; ปิยาภรณ์ สุภัคดำรงกุล Abstract: The focus of this study was on production, characterization and application of an extracellular lipase from Bacillus sp., isolated from soil. Thirty six bacterial strains were screened by activity on lipase test medium and then further tested to determine their activity by using an agar plate containing tributyrin as an indicative substrate. Therefore, a strain HCU3-2 was isolated as the best producer and finally identified as Bacillus sp. HCU3-2. The maximum lipase production was obtained palm fatty acid distillated from palm oil refining process at concentration of 2% (v/v) of basal medium (84.69 +- 0.48 U/mg), followed in order by wastewater from palm oil refining process (46.86 +- 0.75 U/mg). The higher lipase production was obtained when ammonium sulfate was used at concentration of 0.5% (v/v) of basal medium (90.71+-0.11 U/mg). The optimum temperature and pH for lipase production were 45 °C and pH 7, respectively. Zymogram assay of crude enzyme on SDS-PAGE presented one band with lipase activity of molecular weight of 66 kDa. The optimum pH and temperature for activity were pH 11 and 55 °C respectively. The enzyme was stable in the pH range 9-11 for 60 min and at 40-55 °C for 60 min. Higher activity was observed in the presence of surfactants, emulsifier, Ca2+, Mg2+, Zn2+ ions and strongly inhibited lipase activity by DTT. The enzyme exhibited significant stability in the presence of commercial detergents and oxidizing agents. The remarkable resistance capability of the lipase makes it a potential additive for detergent formulation and other industrial applications. Description: Proceedings of the 7th AOHUPO Congress and 9th International Symposium of the Protein Society of Thailand, August 6-8, 2014; Bangkok, Thailand; 2014. p. 259-68. Wed, 01 Jan 2014 00:00:00 GMT https://has.hcu.ac.th/xmlui/handle/123456789/6216 2014-01-01T00:00:00Z https://has.hcu.ac.th/xmlui/handle/123456789/6040 Title: เรื่องน่ารู้เกี่ยวกับฟลูออไรด์ Authors: เกษม พลายแก้ว; Kasem Plaikaew Wed, 01 Jan 2003 00:00:00 GMT https://has.hcu.ac.th/xmlui/handle/123456789/6040 2003-01-01T00:00:00Z https://has.hcu.ac.th/xmlui/handle/123456789/6039 Title: หญิงตั้งครรภ์ต้องระวังทอกโซพลาสโมซิส Authors: สุภาภรณ์ วรรณภิญโญชีพ; Supaporn Wannapinyosheep Wed, 01 Jan 2003 00:00:00 GMT https://has.hcu.ac.th/xmlui/handle/123456789/6039 2003-01-01T00:00:00Z https://has.hcu.ac.th/xmlui/handle/123456789/6036 Title: มารู้จักสารก่อมะเร็ง “ไดออกซิน” กันเถอะ Authors: สุวรรณี สายสิน; Suwannee Saisin Wed, 01 Jan 2003 00:00:00 GMT https://has.hcu.ac.th/xmlui/handle/123456789/6036 2003-01-01T00:00:00Z