https://has.hcu.ac.th/jspui/handle/123456789/2181 2026-04-07T18:56:02Z 2026-04-07T18:56:02Z Kamonrat Pangarean Thidarat Chiewwet Karakade Moonkhum Piyaporn Supakdamrongkul ปิยาภรณ์ สุภัคดำรงกุล https://has.hcu.ac.th/jspui/handle/123456789/5234 2026-02-25T06:53:50Z 2014-01-01T00:00:00Z

Optimization of Lipase Production from Lipase-Producing Bacteria in Soil Using Waster of Industrial Vegetable Oil Production Kamonrat Pangarean; Thidarat Chiewwet; Karakade Moonkhum; Piyaporn Supakdamrongkul; ปิยาภรณ์ สุภัคดำรงกุล Lipase production by the isolate HCU07 was optimized by varying environmental and nutritional conditions in culture medium containing different used vegetable oils and the waste of industrial vegetable oil production at a concentration of 2% (v/v) of basal medium with shaking at 200 rpm for 24 h at room temperature (27-30 °C). The maximum lipase production was obtained using palm fatty acid distillates from oil refining process (16.57+-0.62 U/mg), followed in order by used soybean oil (16.16+-0.77 U/mg), used palm oil (14.29+-0.52 U/mg) and washed water from oil refining process (12.99+-0.22 U/mg), respectively. The highest lipase activity (27.57+-0.33 U/mg) was obtained when soytone was used at concentration of 0.5% (v/v) of basal medium. The optimal pH and temperature for lipase production were pH 7 (28.03+-0.12 U/mg) and room temperature (27-30 °C) (28.53+-0.12C U/mg), respectively. Zymogram assay of crude enzyme on SDS-PAGE presented one bands with lipase activity of molecular weight of 21.5 kDa. The enzyme has good potential for the hydrolysis of used vegetable oils and the waste of industrial vegetable oil production, which are industrially and economically important for industrial applications. การประชุมวิชาการวิทยาศาสตร์และเทคโนโลยีแห่งประเทศไทย ครั้งที่ 40 (Proceedings of the 40th Congress on Science and Technology of Thailand) (STT40) “วิทยาศาสตร์และเทคโนโลยี สู่วิถีพัฒนาอาเซียน” (Science and Technology towards ASEAN Development), December 2-4, 2014 Hotel Pullman, Khon Kaen Raja Orchid, Organized by: The Science Society of Thailand under the Patronage of His Majesty the King in association with the Faculty of Science, Khon Kaen University, Khon Kaen, Thailand : 540-545.

2014-01-01T00:00:00Z Piyaporn Supakdamrongkul Pimpawee Prasong Kattaleya Gitram ปิยาภรณ์ สุภัคดำรงกุล https://has.hcu.ac.th/jspui/handle/123456789/5228 2026-02-23T06:52:03Z 2014-01-01T00:00:00Z

Production and Characterizations of Thermostable Alkaline Lipase from a Newly Isolated Pseudomonas SP. HCU2-1 Suitable for Detergent Formulation Piyaporn Supakdamrongkul; Pimpawee Prasong; Kattaleya Gitram; ปิยาภรณ์ สุภัคดำรงกุล Lipase-producing bacteria were successfully isolated and selected thorough ecological niches and soil samples in which the activity was expected to be found. Thirty five bacterial strains were screened by activity on lipase test medium, and then further tested to determine their activity by using an agar plate containing tributyrin as an indicative substrate. Consequently, a strain HCU2-1 was isolated as the best produces and finally identified as Pseudomonas SP. HCU2-1. Lipase production by Pseudomonas SP. HCU2-1 was optimized by varying environmental and nutritional conditions. The maximum lipase production was obtained using wastewater from palm oil refining process at concentration of 2% (v/v) of basal medium (70.29+-).09 U/mg), followed in order by wastewater from soybean oil refining process (62.59+-0.38 U/mg) and crude acid oil from soybean oil refining process (62.48+-0.43 U/mg). respectively. The highest lipase activity (77.14+-0.33 U/mg) was obtained when peptone was used at concentration of 0.5% (v/v) of basal medium. The optimum temperature and pH for lipase production were 40 °C and pH 7, respectively. Zymogram assay of crude enzyme on SDS-PAGE presented one band with lipase activity of molecular weight of 45kDa. The optimum pH and temperature for activity were pH 11 and 60 °C, respectively. The enzyme was stable in the pH range 9-11 for 60 min and at 40-60 °C for 60 min. Higher activity was observed in the presence of surfactants, Ca2+, Mg2+, Mn2+ ions and strongly inhibited lipase activity by DTT and PMSF. The enzyme exhibited significant stability in the presence of commercial detergents and oxidizing agents. The remarkable resistance capability of the lipase makes it a potential additive for detergent formulation and other industrial applications. การประชุมวิชาการวิทยาศาสตร์และเทคโนโลยีแห่งประเทศไทย ครั้งที่ 40 (Proceedings of the 40th Congress on Science and Technology of Thailand) (STT40) “วิทยาศาสตร์และเทคโนโลยี สู่วิถีพัฒนาอาเซียน” (Science and Technology towards ASEAN Development), December 2-4, 2014 Hotel Pullman, Khon Kaen Raja Orchid, Organized by: The Science Society of Thailand under the Patronage of His Majesty the King in association with the Faculty of Science, Khon Kaen University, Khon Kaen, Thailand : 524-533.

2014-01-01T00:00:00Z Pornpimon Kanjanavas Panuphat Sinwisarn Suchaya Jitrakthai Thanthida Nernchai Rapeepta Wongsinhakul Kosum Chansiri พรพิมล กาญจนวาศ โกสุม จันทร์ศิริ https://has.hcu.ac.th/jspui/handle/123456789/5219 2026-02-22T07:43:15Z 2015-01-01T00:00:00Z

Screening and Producing of Polyhydroxyalkanoated (PHAs) from Soil Bacteria Pornpimon Kanjanavas; Panuphat Sinwisarn; Suchaya Jitrakthai; Thanthida Nernchai; Rapeepta Wongsinhakul; Kosum Chansiri; พรพิมล กาญจนวาศ; โกสุม จันทร์ศิริ PHAs granular accumulating bacteria cell isolated from Donmuang landfill soil, Bangkok, Thailand, three of isolates, DM12-22, DM16-11 and DM30-11 shown high intracellular PHAs accumulation when cultured in mineral salt medium supplemented with molasses as carbon source and then screened by using of Sudan Back B straining technique. After pure culture in mineral salt broth fermentor, growth rate and PHAs accumulation were investigated. For growth rate, DM12-22 showed highest growth rate within 48 hr of culturing period. In addition, for PHAs accumulation, DM30-11 showed highest PHAs compared with DM-16-11 and DM12-22, were 0.092 g/l-1 (0.49% of dry weight), 0.065 g/l-1 (0.41% of dry weight), and 0.037 g/l-1 (0.18% of dry weight), respectively. IBCELC International Biotechnology, Chemical Engineering and Life Science Conference, July 2015 Hokkaido, Japan : 868-876.

2015-01-01T00:00:00Z Chutapa Kunsook Alisa Soontornwat Supaporn Wannapinyosheep Dechavudh Nityasuddhi Vipawan Vitayakritsirikul Vipawan Vitayakritsirikul ชุตาภา คุณสุข อลิษา สุนทรวัฒน์ สุภาภรณ์ วรรณภิญโญชีพ เดชาวุธ นิตยสุทธิ วิภาวรรณ วิทยกฤตศิริกุล พรพิมล กาญจนวาศ https://has.hcu.ac.th/jspui/handle/123456789/5212 2026-02-21T02:16:12Z 2015-01-01T00:00:00Z

Biodiversity of Snakeskin Gourami (Trichogaster pectoralis) in Thailand Chutapa Kunsook; Alisa Soontornwat; Supaporn Wannapinyosheep; Dechavudh Nityasuddhi; Vipawan Vitayakritsirikul; Vipawan Vitayakritsirikul; ชุตาภา คุณสุข; อลิษา สุนทรวัฒน์; สุภาภรณ์ วรรณภิญโญชีพ; เดชาวุธ นิตยสุทธิ; วิภาวรรณ วิทยกฤตศิริกุล; พรพิมล กาญจนวาศ The biodiversity of Snakeskin gourami (Trichogaster pectoralis) from 3 locations (Samutprakan, Samutsakorn and Ayutthaya province), Thailand was investigated by morphological and molecular techniques. The study of morphology showed the similarity between Samutprakan province’s fish and Samutsakorn province’s fish was represented three of characters such as Tiger banded fish, single black banded in middle fish and un-banded fish. Although, Ayutthaya province’s fish gad only one character was Tiger banded fish. The molecular techniques confirm the morphological analyzed by amplification of cytochrome b gene that showed Samutprakan province’s fish was similar with Samutsakorn province’s fish than Ayutthaya province’s fish. IBCELC International Biotechnology, Chemical Engineering and Life Science Conference, July 2015 Hokkaido, Japan : 861-867.

2015-01-01T00:00:00Z