Medical Technology - Articles Journals

Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis

2026-04-15T01:10:25Z

Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis Witthaya Anamnart; Pewpan Maleewong Intapan; Attarat Pattanawongsa; Pennapa Chamavit; Supreecha Kaewsawat; Wanchai Maleewong; วิทยา อานามนารถ; ผิวพรรณ มาลีวงษ์; อรรถรัตน์ พัฒนวงศา; เพ็ญนภา ชมะวิต; สุปรีชา แก้วสวัสดิ์; วันชัย มาลีวงษ์ Dispersion or dilution of stool by water from heavy rainfall may affect Strongyloides stercoralis free-living development producing infective filariform larvae (FL). This study examined effect of water dilution of stool on survival of S. stercoralis free-living development. One g of stool was prepared in water so that its soluble component was diluted sequentially from 1:2 to 1:480. Three dishes were used to compare FL production in three culture conditions: stool suspension, stool sediment deposited in soil and isolated rhabditiform larvae (RhL) deposited in soil. The fourth dish was for developmental observation of RhL into free-living stages. Numerous FL were generated from undiluted or 1:2 diluted stool and stool sediment placed on soil. However, starting from dilution 1:5, FL production continuously decreased in both stool suspensions and stool sediments placed on soil. RhL isolated from stool dilutions placed on soil gave rise to few FL. Worm mating were seen at 24-30 hours in dilutions 1:20-1:120 only. Highest numbers of FL from indirect free-living cycle were 1/3 of those from control. FL production decreased as stool dilution increased and reached zero production at 1:160 dilution. Rainfall may disperse or dilute stool so that nutritional supplement for S. stercoralis free-living development is insufficient. สามารถเข้าถึงบทความฉบับเต็ม (Full Text) ได้ที่ : https://www.nature.com/articles/srep10749

FCGR3B gene frequencies among ethnic Thai blood donors from a regional hospital in Eastern Thailand

2026-04-15T01:11:11Z

FCGR3B gene frequencies among ethnic Thai blood donors from a regional hospital in Eastern Thailand Chalunda Kongmaroeng; Kesorn Kumkaen; ชลันดา กองมะเริง; เกษร คำแก่น The FCGR3B gene encodes three human neutrophil antigens which consist of HNA-1a, HNA-1b, and HNA-1c. These antigens are encoded by three alleles in the FCGR3B locus: FCGR3B*01, FCGR3B*02, and FCGR3B*03 alleles, respectively. The frequencies of FCGR3B alleles have been reported in different ethnic populations. This study compared the FCGR3B gene frequencies among 230 unrelated healthy Eastern Thai blood donors in Rayong hospital with the previously published studies. The polymerase chain reaction-sequence-specific primers method was performed to determine FCGR3B genotypes. The results showed that the allele frequencies of FCGR3B*01, FCGR3B*02, and FCGR3B*03 were 0.722, 0.274, and 0.009, respectively. The FCGR3B*01 and FCGR3B*02 frequencies found in the Eastern Thais were similar to the previous reports investigating in Northern Thais, Chinese Han, Taiwanese, and Japanese populations. Interestingly, our data showed statistically significant difference (P < 0.05) to Central Thais, Korean, Indian, Turkish, Australian, Tunisian, American, German, and Italian populations. In addition, one FCGR3Bnull , which represents a gene deletion, was also found in this study. This information is important not only for the assessment of neutrophil antibody-mediated clinical conditions and for disease association studies but also for anthropological studies. สามารถเข้าถึงบทความฉบับเต็ม (Full Text) ได้ที่ : https://pubmed.ncbi.nlm.nih.gov/25626603/

Determining a New Formula for Calculating Low-Density Lipoprotein Cholesterol: Data Mining Approach

2026-04-15T01:12:00Z

Determining a New Formula for Calculating Low-Density Lipoprotein Cholesterol: Data Mining Approach Prabhop Dansethakul; Lalin Thapanathamchai; Sarawut Suttirat; Apilak Worachartcheewan; Phannee Pidetcha; ประภพ ด่านเศรษฐกุล; ลลิล ฐาปนธรรมชัย; ศราวุธ สุทธิรัตน์; อภิลักษณ์ วรชาติชีวัน; พรรณี พิเดช Low-density lipoprotein cholesterol (LDL-C) is a risk factor of coronary heart diseases. The estimation of LDL-C (LDL-Cal) level was performed using Friedewald's equation for triglyceride (TG) level less than 400 mg/dL. Therefore, the aim of this study is to generate a new formula for LDL-Cal and validate the correlation coefficient between LDL-Cal and LDL-C directly measured (LDL-Direct). A data set of 1786 individuals receiving annual medical check-ups from the Faculty of Medical Technology, Mahidol University, Thailand in 2008 was used in this study. Lipid profiles including total cholesterol (TC), TG, high-density lipoprotein cholesterol (HDL-C) and LDL-C were determined using Roche/Hitachi modular system analyzer. The estimated LDL-C was obtained using Friedewald's equation and the homogenous enzymatic method. The level of TG was divided into 6 groups (TG<200, <300, <400, <500, <600 and < 1000 mg/dL) for constructing the LDL-Cal formula. The pace regression model was used to construct the candidate formula for the LDL-Cal and determine the correlation coefficient (r) with the LDL-Direct. The candidate LDL-Cal formula was generated for 6 groups of TG levels that displayed well correlation between LDL-Cal and LDL-Direct. Interestingly, The TG level was less than 1000 mg/dL, the regression model was able to generate the equation as shown as strong r of 0.9769 with LDL-Direct. Furthermore, external data set (n = 666) with TG measurement (36-1480 mg/dL) was used to validate new formula which displayed high r of 0.971 between LDL-Cal and LDL-direct. This study explored a new formula for LDL-Cal which exhibited higher r of 0.9769 and far beyond the limitation of TG more than 1000 mg/dL and potential used for estimating LDL-C in routine clinical laboratories. สามารถเข้าถึงบทความฉบับเต็ม (Full Text) ได้ที่ : https://pmc.ncbi.nlm.nih.gov/articles/PMC4652637/

Computational analyses of curcuminoid analogs against kinase domain of HER2

2026-04-15T01:13:13Z

Computational analyses of curcuminoid analogs against kinase domain of HER2 Wannarat Yim-im; Orathai Sawatdichaikul; Suwanna Samesri; Natharinee Horata; Wanwimon Mokmak; Sissades Tongsima; Apichart Suksamrarn; Kiattawee Choowongkomon; วรรณรัตน์ ยิ้มอิ่ม; อรไท สวัสดิชัยกุล; สุวรรณา เสมศรี; ณัฐริณี หอระตะ; วรรณวิมล หมอกมาก; ศิษเฎศ ทองสิมา; อภิชาต สุขสําราญ; เกียรติทวี ชูวงศ์โกมล Background: Human epidermal growth factor receptor 2 (HER2) has an important role in cancer aggressiveness and poor prognosis. HER2 has been used as a drug target for cancers. In particular, to effectively treat HER2-positive cancer, small molecule inhibitors were developed to target HER2 kinase. Knowing that curcumin has been used as food to inhibit cancer activity, this study evaluated the efficacy of natural curcumins and curcumin analogs as HER2 inhibitors using in vitro and in silico studies. The curcumin analogs considered in this study composed of 4 groups classified by their core structure, β-diketone, monoketone, pyrazole, and isoxazole. Results: In the present study, both computational and experimental studies were performed. The specificity of curcumin analogs selected from the docked results was examined against human breast cancer cell lines. The screened curcumin compounds were then subjected to molecular dynamics simulation study. By modifying curcumin analogs, we found that protein-ligand affinity increases. The benzene ring with a hydroxyl group could enhance affinity by forming hydrophobic interactions and the hydrogen bond with the hydrophobic pocket. Hydroxyl, carbonyl or methoxy group also formed hydrogen bonds with residues in the adenine pocket and sugar pocket of HER2-TK. These modifications could suggest the new drug design for potentially effective HER2-TK inhibitors. Two outstanding compounds, bisdemethylcurcumin (AS-KTC006) and 3,5-bis((E)-3,4-dimethoxystyryl)isoxazole (AS-KTC021 ),were well oriented in the binding pocket almost in the simulation time, 30 ns. This evidence confirmed the results of cell-based assays and the docking studies. They possessed more distinguished interactions than known HER2-TK inhibitors, considering them as a promising drug in the near future. Conclusions: The series of curcumin compounds were screened using a computational molecular docking and followed by human breast cancer cell lines assay. Both AS-KTC006 and AS-KTC021 could inhibit breast cancer cell lines though inhibiting of HER2-TK. The intermolecular interactions were confirmed by molecular dynamics simulation studies. This information would explore more understanding of curcuminoid structures and HER2-TK.