Development and assessment of ELISA for serodiagnosis of HIV-infection.

Pramote Thongkrajai; R. Kaewmart; ปราโมทย์ ทองกระจาย

dc.contributor.author	Pramote Thongkrajai
dc.contributor.author	R. Kaewmart
dc.contributor.author	ปราโมทย์ ทองกระจาย
dc.contributor.other	Huachiew Chalermprakiet University. Faculty of Public and Environmental Health	th
dc.contributor.other	Khon Kaen University. Faculty of Medicine	th
dc.date.accessioned	2024-01-06T12:10:43Z
dc.date.available	2024-01-06T12:10:43Z
dc.date.issued	1998
dc.identifier.citation	Southeast Asian J Trop Med Public Health 29,4 (December 1998) : 763-766	th
dc.identifier.uri	https://has.hcu.ac.th/jspui/handle/123456789/1607
dc.description	สามารถเข้าถึงบทความฉบับเต็มได้ที่ (Full text) https://www.tm.mahidol.ac.th/seameo/1998-29-4/1998-29-4-763.pdf
dc.description.abstract	A home-made ELISA for serodiagnosis of HIV-infection was developed. It made use of the HIV viral lysate to coat on ELISA microplates. The purpose was to establish an ELISA for serodiagnosis of HIV-infection. The newly-developed ELISA, "H-ELISA", was applied to test 792 samples of HIV-positive serum as confirmed by Western blot. All 792 samples were positive by H-ELISA. It was also applied to test 540 samples of normal sera obtained from different laboratories in Srinagarind Hospital. A number 530 normal sera was negative, 8 samples were positive and confirmed by Western blot and 2 samples were false positive. It was concluded that the H-ELISA possessed 100% sensitivity with a false positive rate of 2/532(0.38%). The H-ELISA, the cost/test was less than 5 bahts, appears to be promising for substitution of imported commercial kits.	th
dc.language.iso	en_US	th
dc.subject	Enzyme-linked Immunosorbent Assay	th
dc.subject	เอนไซม์ลิงค์อิมมูโนซอร์เบนท์แอสเส	th
dc.subject	HIV-infection	th
dc.subject	การติดเชื้อเอชไอวี	th
dc.subject	Serodiagnosis	th
dc.subject	การวิฉัยตรวจโดยซีรัม	th
dc.title	Development and assessment of ELISA for serodiagnosis of HIV-infection.	th
dc.type	Article	th