dc.contributor.author |
Pramote Thongkrajai |
|
dc.contributor.author |
R. Kaewmart |
|
dc.contributor.author |
ปราโมทย์ ทองกระจาย |
|
dc.contributor.other |
Huachiew Chalermprakiet University. Faculty of Public and Environmental Health |
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dc.contributor.other |
Khon Kaen University. Faculty of Medicine |
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dc.date.accessioned |
2024-01-06T12:10:43Z |
|
dc.date.available |
2024-01-06T12:10:43Z |
|
dc.date.issued |
1998 |
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dc.identifier.citation |
Southeast Asian J Trop Med Public Health 29,4 (December 1998) : 763-766 |
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dc.identifier.uri |
https://has.hcu.ac.th/jspui/handle/123456789/1607 |
|
dc.description |
สามารถเข้าถึงบทความฉบับเต็มได้ที่ (Full text)
https://www.tm.mahidol.ac.th/seameo/1998-29-4/1998-29-4-763.pdf |
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dc.description.abstract |
A home-made ELISA for serodiagnosis of HIV-infection was developed. It made use of the HIV viral lysate to coat on ELISA microplates. The purpose was to establish an ELISA for serodiagnosis of HIV-infection. The newly-developed ELISA, "H-ELISA", was applied to test 792 samples of HIV-positive serum as confirmed by Western blot. All 792 samples were positive by H-ELISA. It was also applied to test 540 samples of normal sera obtained from different laboratories in Srinagarind Hospital. A number 530 normal sera was negative, 8 samples were positive and confirmed by Western blot and 2 samples were false positive. It was concluded that the H-ELISA possessed 100% sensitivity with a false positive rate of 2/532(0.38%). The H-ELISA, the cost/test was less than 5 bahts, appears to be promising for substitution of imported commercial kits. |
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dc.language.iso |
en_US |
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dc.subject |
Enzyme-linked Immunosorbent Assay |
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dc.subject |
เอนไซม์ลิงค์อิมมูโนซอร์เบนท์แอสเส |
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dc.subject |
HIV-infection |
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dc.subject |
การติดเชื้อเอชไอวี |
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dc.subject |
Serodiagnosis |
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dc.subject |
การวิฉัยตรวจโดยซีรัม |
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dc.title |
Development and assessment of ELISA for serodiagnosis of HIV-infection. |
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dc.type |
Article |
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