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Development and assessment of ELISA for serodiagnosis of HIV-infection.

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dc.contributor.author Pramote Thongkrajai
dc.contributor.author R. Kaewmart
dc.contributor.author ปราโมทย์ ทองกระจาย
dc.contributor.other Huachiew Chalermprakiet University. Faculty of Public and Environmental Health th
dc.contributor.other Khon Kaen University. Faculty of Medicine th
dc.date.accessioned 2024-01-06T12:10:43Z
dc.date.available 2024-01-06T12:10:43Z
dc.date.issued 1998
dc.identifier.citation Southeast Asian J Trop Med Public Health 29,4 (December 1998) : 763-766 th
dc.identifier.uri https://has.hcu.ac.th/jspui/handle/123456789/1607
dc.description สามารถเข้าถึงบทความฉบับเต็มได้ที่ (Full text) https://www.tm.mahidol.ac.th/seameo/1998-29-4/1998-29-4-763.pdf
dc.description.abstract A home-made ELISA for serodiagnosis of HIV-infection was developed. It made use of the HIV viral lysate to coat on ELISA microplates. The purpose was to establish an ELISA for serodiagnosis of HIV-infection. The newly-developed ELISA, "H-ELISA", was applied to test 792 samples of HIV-positive serum as confirmed by Western blot. All 792 samples were positive by H-ELISA. It was also applied to test 540 samples of normal sera obtained from different laboratories in Srinagarind Hospital. A number 530 normal sera was negative, 8 samples were positive and confirmed by Western blot and 2 samples were false positive. It was concluded that the H-ELISA possessed 100% sensitivity with a false positive rate of 2/532(0.38%). The H-ELISA, the cost/test was less than 5 bahts, appears to be promising for substitution of imported commercial kits. th
dc.language.iso en_US th
dc.subject Enzyme-linked Immunosorbent Assay th
dc.subject เอนไซม์ลิงค์อิมมูโนซอร์เบนท์แอสเส th
dc.subject HIV-infection th
dc.subject การติดเชื้อเอชไอวี th
dc.subject Serodiagnosis th
dc.subject การวิฉัยตรวจโดยซีรัม th
dc.title Development and assessment of ELISA for serodiagnosis of HIV-infection. th
dc.type Article th


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