Please use this identifier to cite or link to this item: https://has.hcu.ac.th/jspui/handle/123456789/2053
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dc.contributor.authorPreeyaporn Monatrakul Sreepian-
dc.contributor.authorPanthip Rattanasinganchan-
dc.contributor.authorApichai Sreepian-
dc.contributor.authorปรียาภรณ์ โมนะตระกูล ศรีเพียร-
dc.contributor.authorปานทิพย์ รัตนศิลป์กัลชาญ-
dc.contributor.authorอภิชัย ศรีเพียร-
dc.contributor.otherRangsit University. Faculty of Medical Technologyth
dc.contributor.otherHuachiew Chalermprakiet University. Faculty of Medical Technologyth
dc.contributor.otherRangsit University. Faculty of Medical Technologyth
dc.date.accessioned2024-04-14T13:49:33Z-
dc.date.available2024-04-14T13:49:33Z-
dc.date.issued2023-
dc.identifier.citationSaudi Pharmaceutical Journal 31, 6, (June 2023) : 1094-1103th
dc.identifier.otherhttps://doi.org/10.1016/j.jsps.2023.03.020-
dc.identifier.urihttps://has.hcu.ac.th/jspui/handle/123456789/2053-
dc.descriptionสามารถเข้าถึงบทความฉบับเต็มได้ที่ https://doi.org/10.1016/j.jsps.2023.03.020th
dc.description.abstractThe increasing incidence of methicillin-resistant S. aureus is a major public health concern. Recently, the performance of Citrus hystrix essential oil (CHEO) has been shown to contain broad-spectrum antibacterial activity. Therefore, this study aims to determine the antibacterial activity of CHEO alone and in combination with gentamicin against panels of clinical isolates of methicillin-susceptible S. aureus (MSSA, n = 45) and methicillin-resistant S. aureus (MRSA, n = 40). Antibiotic susceptibility testing revealed multidrug-resistant (MDR) patterns among 3 MSSA isolates and 39 MRSA isolates, indicating that the clinical MRSA isolates were associated with MDR (p < 0.05). For the drug resistant isolates, resistance was observed toward most antibiotics, except for chloramphenicol, trimethoprim-sulfamethoxazole, linezolid, and vancomycin. Antibacterial screening by disk diffusion demonstrated that CHEO alone had certain antibacterial activity toward all MSSA isolates (IZD: 16.0 ± 4.7 mm) and MRSA isolates (IZD: 16.5 ± 4.2 mm) (p > 0.05). The MIC values of CHEO are 18.3 ± 6.1 mg/mL in MSSA isolates and 17.9 ± 6.9 mg/mL in MRSA isolates (p > 0.05). The antibacterial activity of CHEO demonstrated the bactericidal effect with MIC index 1.0-1.4. Time-killing kinetics revealed that CHEO at 1 × MIC completely killed MSSA and MRSA within 12 h. Moreover, the checkerboard titration demonstrated the synergistic and additive interactions of CHEO with gentamicin with FIC index 0.012-0.625. CHEO against human epidermal keratinocyte; HaCaT cell line demonstrated the IC50 value at 2.15 mg/mL. The use of CHEO as an alternative antibacterial agent would reduce the emergence of resistant bacteria, especially MDR MRSA.th
dc.language.isoen_USth
dc.subjectฤทธิ์ต้านเชื้อแบคทีเรียth
dc.subjectAntibacterial activityth
dc.subjectพิษต่อเซลล์th
dc.subjectCytotoxicityth
dc.subjectมะกรูดth
dc.subjectCitrus hystrixth
dc.subjectการดื้อยาหลายชนิดth
dc.subjectMultidrug resistanceth
dc.subjectMethicillin-Resistant Staphylococcus Aureusth
dc.titleAntibacterial efficacy of Citrus hystrix (makrut lime) essential oil against clinical multidrug-resistant methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolatesth
dc.typeArticleth
Appears in Collections:Medical Technology - Artical Journals

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