Development of Aqueous Formulation Containing the Extracted Mangiferin

Abstract

Mangiferin, a polyphenol of C-glycosylxanthone, exhibits various bioactivities with poor aqueous solubility. It is known as a potent antioxidant, which leads to remarkable UV protection and anti-aging properties. Mangiferin can be found in many plant species, among which the mango leaf is one of the primary sources. From our study, the extraction yield of mangifein obtained from the leaves of Mangifera indica L. variety Nam Doc Mai was 3.17% with 95.02% ± 0.064 purity (HPTLC analysis). The solubility of mangiferin in the studied pure solvents arranging in descending order were ethoxydiglycol, dimethyl isosorbide, polyethylene glycol 400, polyethylene glycol 600, propylene glycol, dipropylene glycol, glycerin, isopentyldiol, methanol, ethanol and water, whereas the addition of the solvent in water could increase the aqueous solubility of mangiferin. In several cases, the solubility was apparently higher than that dissolved in its pure solvent state. The log-linear solubility model for the cosolvent system was used to calculate the volume fractions of the selected solvents needed to solubilize mangiferin content at the twenty times of the IC50 against DPPH radicals. In conclusion, the developed aqueous formulation contained 0.5% w/v of mangiferin and 20% w/v of polyethylene glycol 600 or dipropylene glycol as a solubilizer in water.