1. Huachiew Chalermprakiet University
  2. มหาวิทยาลัยหัวเฉียวเฉลิมพระเกียรติ
  3. คณะเทคนิคการแพทย์
  4. Medical Technology - Artical Journals
Please use this identifier to cite or link to this item: https://has.hcu.ac.th/jspui/handle/123456789/3758
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dc.contributor.authorPanthip Rattanasinganchan-
dc.contributor.authorKittipat Sopitthummakhun-
dc.contributor.authorKent Doi-
dc.contributor.authorXuzhen Hu-
dc.contributor.authorD. Michael Payne-
dc.contributor.authorTrairak Pisitkun-
dc.contributor.authorAsada Leelahavanichkul-
dc.contributor.authorปานทิพย์ รัตนศิลป์กัลชาญ-
dc.contributor.authorกิตติพัฒน์ โสภิตธรรมคุณ-
dc.contributor.authorไตรรักษ์ พิสิษฐ์กุล-
dc.contributor.authorอัษฎาศ์ ลีฬหวนิชกุล-
dc.contributor.otherHuachiew Chalermprakiet University. Faculty of Medical Technologyen
dc.contributor.otherHuachiew Chalermprakiet University. Faculty of Science and Technologyen
dc.contributor.otherNational Institute of Diabetes and Digestive and Kidney Diseases. University Kidney Disease Branchen
dc.contributor.otherHuachiew Chalermprakiet University. Faculty of Medical Technologyen
dc.contributor.otherChulalongkorn University. Faculty of Medicineen
dc.contributor.otherChulalongkorn University. Faculty of Medicineen
dc.contributor.otherChulalongkorn University. Faculty of Medicineen
dc.date.accessioned2025-03-22T12:56:55Z-
dc.date.available2025-03-22T12:56:55Z-
dc.date.issued2016-
dc.identifier.citationAsian Biomedicine 10, 5 (October 2016) : 491 - 502en
dc.identifier.otherDOI: https://doi.org/10.5372/1905-7415.1005.513-
dc.identifier.urihttps://has.hcu.ac.th/jspui/handle/123456789/3758-
dc.descriptionสามารถเข้าถึงบทความฉบับเต็ม (Full Text) ได้ที่ : https://sciendo.com/article/10.5372/1905-7415.1005.513en
dc.description.abstractBackground: Chronic tubulointerstitial (TI) fibrosis is difficult to diagnose. Urinary exosomes contain many potential biomarkers, and may be useful to identify biomarkers of TI fibrosis. However, a suitable animal model to investigate urinary exosome biomarkers for TI fibrosis has not yet been specifically identified. Objective : To test the suitability of a folic-acid induced rat model of renal injury for identifying potential biomarkers of fibrosis from urinary exosomes. Methods: We employed a rat model of renal injury using a single intraperitoneal injection of folic acid. Urinary exosomes were isolated and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) Results: We demonstrated severe acute kidney injury after folic acid injection by a 6-fold decrease in creatinine clearance at day 2, severe inflammatory cell infiltration in the TI area at day 7, and renal fibrosis at day 14. We collected 24 h urine samples from folic acid-injected rats and vehicle-injected control rats at days 7 and 14, then isolated urinary exosomes using a differential centrifugation method. We found that levels of exosomal marker proteins ALG-2-interacting protein 1 (ALIX; PDCD6IP) and tumor susceptibility gene 101 (TSG101) were higher in folic acid-injected rats than in control rats. LC-MS/MS analysis of urinary exosomal proteins from the folic acid-injected and control rats identified 372 proteins, including various potential TI fibrosis biomarkers Conclusion: We demonstrated the feasibility of using a folic acid-induced rat model of TI fibrosis for urinary proteomic analysis. These rats excreted sufficient urinary exosomes permitting the large-scale analysis and discovery of potential biomarkers of renal fibrosisen
dc.language.isoen_USen
dc.subjectBiomarkersen
dc.subjectตัวบ่งชี้ทางชีวภาพen
dc.subjectKidneys -- Diseasesen
dc.subjectไต – โรคen
dc.subjectChronic kidney diseaseen
dc.subjectโรคไตเรื้อรังen
dc.subjectFolic aciden
dc.subjectกรดโฟลิกen
dc.subjectInterstitial fibrosisen
dc.subjectอินเทอร์สทิเซียลไฟโบรซิสen
dc.subjectเนื้อเยื่อพังผืดระหว่างเซลล์กล้ามเนื้อen
dc.subjectProteomicsen
dc.subjectโปรตีโอมิกส์en
dc.subjectUrinary exosomesen
dc.titleA folic acid-induced rat model of renal injury to identify biomarkers of tubulointerstitial fibrosis from urinary exosomesen
dc.typeArticleen
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