Quantitative Determination of Mangiferin Isolated from Leaves of Mangifera indica L. Variety Nam Doc Mai Using HPTLC and Its DPPH Scavenging Activity

Abstract

Mangiferin (2-beta-D-glucopyranosyl-1,3,6,7-tetrahydroxyxanthen-9-one) was isolated from Leaves of Mangifera indica L. variety Nam Doc Mai. It showed a potent scavenging activity on DPPH redical with an IC50 value of 6.38 μg/ml. A simple and cost-effective high- performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative determination of mangiferin. The method was performed on silica gel 60 F254 pre-coated plate with ethyl acetate-acetone-formic acid-water 8:2:1:1 (v/v) as mobile phase. Detection and quantitation was achieved by densitometric scanning at the wavelength of 320 nm. The results from validation method indicated that the proposed HPTLC method provided a good linearity at the range of 1.0 to 3.0 μg/band, accuracy (107.89 ± 0.29%), precision (intra-day RSD 0.51-1.71% and inter-day RSD 1.52%), and specificity (sharp peak at Rf value of 0.48).