Comparative Effects of Brassinosteroid and Brassinosteroid Mimic on Improving Photosynthesis, Lipid Peroxidation, and Rice Seed Set under Heat Stress

Abstract

The application of brassinosteroids (BRs) has been reported to alleviate heat stress. This study investigated the effect of 7,8-dihydro-8α-20-hydroxyecdysone (DHECD)—a BR mimic—by comparison with 24-epibrassinolide (EBR) on the changes in photosynthetic performance, lipid peroxidation, and rice seed set. The results demonstrated that 10−8 M EBR and 10−7 M DHECD had the best actions to counteract the lethal heat temperature of 47 °C for 2 h indicated by a reduction in the number of wilted leaves and an increase in the relative water content and leaf greenness. Moreover, plants treated with EBR or DHECD were exposed to high day/night temperatures of 40/30 °C for 7 days. EBR-treated and DHECD-treated plants showed a high shoot fresh weight, leaf area, chlorophyll content, and carotenoid content. High temperature significantly decreased the leaf net CO2 assimilation rate as well as increased lipid peroxidation. The application of EBR and DHECD maintained the high level of the net CO2 assimilation rate by increasing the stomatal conductance and photochemical quenching. On the other hand, EBR and DHECD decreased the intracellular CO2 content and non-photochemical quenching leading to enhance photosynthesis under heat stress. EBR-treated and DHECD-treated plants significantly reduced their malondialdehyde and hydrogen peroxide contents as well as increasing their total soluble sugar contents. Moreover, BR treatments increased the filled seed of rice. This study confirmed that DHECD—a BR mimic—has activities of heat stress alleviation similar to EBR.