Please use this identifier to cite or link to this item: https://has.hcu.ac.th/jspui/handle/123456789/1785
Title: Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifca isofavones: a sustainable approach for increasing health-bioactive constituents
Authors: Fonthip Makkliang
Boondaree Siriwarin
Gorawit Yusakul
Suppalak Phaisan
Attapon Sakdamas
Natthapon Chuphol
Waraporn Putalun
Seiichi Sakamoto
ฝนทิพย์ มากเกลี้ยง
บุณฑรี ศิริวาริน
กรวิทย์ อยู่สกุล
ศุภลักษณ์ ไพศาล
อรรถพล ศักดามาศ
วราภรณ์ ภูตะลุน
Walailak University. School of Languages and General Education
Huachiew Chalermprakiet University. Faculty of Pharmaceutical Sciences
Walailak University. School of Pharmacy
Walailak University. School of Pharmacy
Walailak University. School of Pharmacy
Prince of Songkla University. Faculty of Pharmaceutical Sciences
Khon Kaen University. Faculty of Pharmaceutical Sciences
Kyushu University. Graduate School of Pharmaceutical Sciences
Keywords: Biotransformation (Metabolism)
Cellulolytic enzymes
Isoflavonoids
ไอโซฟลาโวนอยด์
Pueraria mirifica
Natural deep eutectic solvent
Issue Date: 2021
Citation: Bioresources and Bioprocessing volume 8, Article number: 76 (2021)
Abstract: The presence of specific gut microflora limits the biotransformation of Pueraria mirifica isoflavone (PMI) glycosides into absorbable aglycones, thus limiting their health benefits. Cellulolytic enzyme-assisted extraction (CAE) potentially solves this issue; however, solvent extraction requires recovery of the hydrophobic products. Here, we established the simultaneous transformation and extraction of PMIs using cellulolytic enzymes and natural deep eutectic solvents (NADESs). The NADES compositions were optimized to allow the use of NADESs as CAE media, and the extraction parameters were optimized using response surface methodology (RSM). The optimal conditions were 14.7% (v/v) choline chloride:propylene glycol (1:2 mol ratio, ChCl:PG) at 56.1 °C for the cellulolytic enzyme (262 mU/mL) reaction in which daidzin and genistin were extracted and wholly transformed to their aglycones daidzein and genistein. The extraction of PMIs using ChCl:PG is more efficient than that using conventional solvents; additionally, biocompatible ChCl:PG enhances cellulolytic enzyme activity, catalyzing the transformation of PMIs into compounds with higher estrogenicity and absorbability.
Description: สามารถเข้าถึงบทความฉบับเต็มได้ที่ https://bioresourcesbioprocessing.springeropen.com/articles/10.1186/s40643-021-00428-9
URI: https://has.hcu.ac.th/jspui/handle/123456789/1785
Appears in Collections:Pharmaceutical Sciences - Artical Journals

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