In vitro antioxidant capacity, total phenolic and flavonoid contents, and wound healing potential in Mangifera indica L. leaf extracts

Abstract

Mangifera indicaL. is a rich source of biological active compounds, prominently Mangiferin, characterized by its glycosylated xanthone structure. This compound is abundant in various edible sources, such as mango, and exhibits diverse biological activities. Extensive investigation into Mangiferin has highlighted antioxidant, anti-inflammatory, antidiabetic, anticancer, and antimicrobial properties. In this study, Mangiferin extract was prepared through maceration in 80% ethanol, yielding 1,628 g from an initial dry leaf mass of 4,234 g of dried leaf material. The ethanolic extract showed notabletorox equivalent antioxidant capacity(TEAC)values for 2,2-diphenyl-1-picrylhydrazyl(DPPH) and2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)assays, measured at 0.32 ± 0.03 and 0.56 ± 0.01 mM Trolox equivalents per gram dry material, respectively. The ferric reducing ability power(FRAP)value was determined to be 6.95 ± 0.40 mM FeSO4 per milligram dry material. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)assays conducted onhumanadult calcium temperature(HaCaT)cell line indicated that concentrations ranging from 3.90 to 31.25 μg/mL did not significantly impact cell viability. Evaluation of the wound healing of the Mangiferin ethanolic extract demonstrates a consistent decrease in wound area for treated cells compared to untreated controls, with significant decreases observed after 48 hours (p< 0.05).These findings support the antioxidant and wound healing properties of the Mangiferin ethanolic extract, underscoring its therapeutic potential in promoting wound repair.