Abstract:
Immunodominant antigens of an approximate molecular mass of 27 kDa (FG 27) were
obtained from an excretory-secretory product of adult Fasciola gigantica by a simple continuous–
elution method. A dot–ELISA using the FG 27 antigen was developed for the detection of specific
antibodies from patients infected with F. gigantica. Control sera were obtained from patients with
other parasitic infections and healthy volunteers. The accuracy, sensitivity, specificity, and positive
and negative predictive values were 98.2%, 100%, 97.4%, 76.9% and 100%, respectively. This dotELISA is a specific, sensitive and easy to perform method for the rapid diagnosis of fascioliasis,
particularly when more complex laboratory tests are unavailable.
