Abstract:
Immunodiagnosis of human trichinellosis papuae by indirect ELISA and immunoblot was developed using Trichinella papuae muscle L1 larva crude worm antigens (TpaL1-CWA) and tested with human sera of trichinellosis papuae (n = 18), healthy control (n = 26) and other parasitic diseases (n = 160). IgG2- and IgG3-indirect ELISAs failed to elicit positive results, total IgG-, IgG1- and IgG4-ELISAs demonstrated sensitivity of 100, 100 and 66.7%, respectively and specificity of 90.3, 89.8 and 66.7%, respectively. Western
blotting of TpaL1-CWA revealed predominant immunoreactive antigens of 31, 45 and 66.2 kDa against total IgG and IgG1 of trichinellosis papuae sera, which, except for 31 kDa antigen, showed cross-reactivity with sera of several other parasitic diseases. Specificity, sensitivity, positive predictive value, and negative predictive value of T. papuae 31 kDa in IgG1-immunoblot were 100%. Mass spectrometry analysis of the 31 kDa antigen revealed the presence (in decreasing order of identity score) of 3-hydroxyacyl-CoA dehydrogenase, GLIPR1-like protein 1, tissue-type plasminogen activator, hypothetical protein T10_8058, and hypothetical protein T10_12289. Future studies should focus on evaluating the most appropriate candidate 31 kDa antigen to prepare recombinant protein for application in trichinellosis papuae immunodiagnosis
