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Detection of carbapenemase producing Enterobacterales by MALDI-TOF mass spectrometry in Samutprakan Hospital, Thailand

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dc.contributor.author Patcharee Kammarnjassadakul
dc.contributor.author Watcharin Rangsipanuratn
dc.contributor.author Sucha Chulsomlee
dc.contributor.author Manop Suttiprapha
dc.contributor.author พัชรี กัมมารเจษฎากุล
dc.contributor.author วัชรินทร์ รังษีภาณุรัตน์
dc.contributor.author สุชา จุลสำลี
dc.contributor.author มานพ สุทธิประภา
dc.contributor.other Huachiew Chalermprakiet University. Faculty of Medical Technology en
dc.contributor.other Huachiew Chalermprakiet University. Faculty of Medical Technology en
dc.contributor.other Huachiew Chalermprakiet University. Faculty of Medical Technology en
dc.contributor.other Samutprakan Hospital. Medical Technology and Clinical Pathology Department en
dc.date.accessioned 2024-08-30T14:02:26Z
dc.date.available 2024-08-30T14:02:26Z
dc.date.issued 2023
dc.identifier.citation Pharm Sci Asia 2023; 50(4), 331-336 en
dc.identifier.other DOI:10.29090/psa.2023.04.23.652
dc.identifier.uri https://has.hcu.ac.th/jspui/handle/123456789/2728
dc.description สามารถเข้าถึงบทความฉบับเต็ม (Full text) ได้ที่ : https://pharmacy.mahidol.ac.th/journal/_files/2023-50-4_8.pdf en
dc.description.abstract A rapid phenotypic carbapenemase-producing Enterobacterales (CPE) detection method was established using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Sixtyfour carbapenem-resistant Enterobacterales (CRE) strains from Samutprakan Hospital were examined using MALDI-TOF MS by the ertapenem hydrolysis method and the susceptibility was compared with a modified carbapenem inactivation method (mCIM). Drug resistance genes were detected by polymerase chain reaction (PCR). The ertapenem drug and bacterial strains were mixed and drug hydrolysis owing to CPE activity was confirmed by specific molecular masses of ertapenem [M+H]+ at 476.5 m/z (±500 ppm), with peak disappearance judged as carbapenemase-positive. The most common CRE species were Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae. From 58 CPE strains, 17 strains of K. pneumoniae (29.3%) harbored blaNDM and blaOXA48- like genes together, while 33 strains of K. pneumoniae (56.9%), 6 strains of E. coli (10.3%) and 1 strain of Ent. cloacae (1.75%) carried blaNDM or blaOXA-48- like genes alone and 1 strain of K. pneumoniae (1.75%) contained blaKPC. After 3 h of incubation with ertapenem, all 58 drug-resistant strains revealed disappearance of the ertapenem-specific waveform peak at 476.5 m/z, whereas 6 strains of CRE (non-CPE) revealed the ertapenemspecific waveform peak. The MALDI-TOF MS and mCIM data were 100% consistent. The MALDI-TOF MS based ertapenem hydrolysis assay was demonstrated as a rapid and accurate method to detect carbapenemase activity of Enterobacterales strains that can be routinely performed in clinical microbiology laboratories. en
dc.language.iso en_US en
dc.subject Carbapenemase en
dc.subject คาร์บาพีนีเมส en
dc.subject Carbapenem-Resistant Enterobacteriaceae en
dc.subject Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry en
dc.title Detection of carbapenemase producing Enterobacterales by MALDI-TOF mass spectrometry in Samutprakan Hospital, Thailand en
dc.type Article en


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