Abstract:
Biosurfactants are valuable microbial amphiphilic molecules with
effective surface-active and biological properties applicable to several
industries and processes. The aim of this study was to optimize nutrients
composition and cultivation conditions for biosurfactant production by
Candida mucifera NJP25. The effects of five variable factors, including
carbon source, nitrogen source, C:N ratio, initial pH and temperature, on
biosurfactant production were investigated using one factor at a time
(OFAT) method. The results revealed that C. mucifera NJP25 produced the
highest biosurfactant (reduced surface tension culture broth 35.50 mN/m
and oil displacement activity of 0.62 cm2
) in optimized medium containing
4.0% sucrose, 0.4% sodium nitrate , C:N ratio approximately 20 with initial
pH 6.5 and incubated at 30°C for 9 days. The highest biosurfactant yield to
0.26 g/L and its culture broth had the critical micelle concentration (CMC)
of 27.48 mg/ml. Our results could provide the practical information for
further large scale production of biosurfactant from yeast.
