A folic acid-induced rat model of renal injury to identify biomarkers of tubulointerstitial fibrosis from urinary exosomes

Panthip Rattanasinganchan; Kittipat Sopitthummakhun; Kent Doi; Xuzhen Hu; D. Michael Payne; Trairak Pisitkun; Asada Leelahavanichkul; ปานทิพย์ รัตนศิลป์กัลชาญ; กิตติพัฒน์ โสภิตธรรมคุณ; ไตรรักษ์ พิสิษฐ์กุล; อัษฎาศ์ ลีฬหวนิชกุล

dc.contributor.author	Panthip Rattanasinganchan
dc.contributor.author	Kittipat Sopitthummakhun
dc.contributor.author	Kent Doi
dc.contributor.author	Xuzhen Hu
dc.contributor.author	D. Michael Payne
dc.contributor.author	Trairak Pisitkun
dc.contributor.author	Asada Leelahavanichkul
dc.contributor.author	ปานทิพย์ รัตนศิลป์กัลชาญ
dc.contributor.author	กิตติพัฒน์ โสภิตธรรมคุณ
dc.contributor.author	ไตรรักษ์ พิสิษฐ์กุล
dc.contributor.author	อัษฎาศ์ ลีฬหวนิชกุล
dc.contributor.other	Huachiew Chalermprakiet University. Faculty of Medical Technology	en
dc.contributor.other	Huachiew Chalermprakiet University. Faculty of Science and Technology	en
dc.contributor.other	National Institute of Diabetes and Digestive and Kidney Diseases. University Kidney Disease Branch	en
dc.contributor.other	Huachiew Chalermprakiet University. Faculty of Medical Technology	en
dc.contributor.other	Chulalongkorn University. Faculty of Medicine	en
dc.contributor.other	Chulalongkorn University. Faculty of Medicine	en
dc.contributor.other	Chulalongkorn University. Faculty of Medicine	en
dc.date.accessioned	2025-03-22T12:56:55Z
dc.date.available	2025-03-22T12:56:55Z
dc.date.issued	2016
dc.identifier.citation	Asian Biomedicine 10, 5 (October 2016) : 491 - 502	en
dc.identifier.other	DOI: https://doi.org/10.5372/1905-7415.1005.513
dc.identifier.uri	https://has.hcu.ac.th/jspui/handle/123456789/3758
dc.description	สามารถเข้าถึงบทความฉบับเต็ม (Full Text) ได้ที่ : https://sciendo.com/article/10.5372/1905-7415.1005.513	en
dc.description.abstract	Background: Chronic tubulointerstitial (TI) fibrosis is difficult to diagnose. Urinary exosomes contain many potential biomarkers, and may be useful to identify biomarkers of TI fibrosis. However, a suitable animal model to investigate urinary exosome biomarkers for TI fibrosis has not yet been specifically identified. Objective : To test the suitability of a folic-acid induced rat model of renal injury for identifying potential biomarkers of fibrosis from urinary exosomes. Methods: We employed a rat model of renal injury using a single intraperitoneal injection of folic acid. Urinary exosomes were isolated and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) Results: We demonstrated severe acute kidney injury after folic acid injection by a 6-fold decrease in creatinine clearance at day 2, severe inflammatory cell infiltration in the TI area at day 7, and renal fibrosis at day 14. We collected 24 h urine samples from folic acid-injected rats and vehicle-injected control rats at days 7 and 14, then isolated urinary exosomes using a differential centrifugation method. We found that levels of exosomal marker proteins ALG-2-interacting protein 1 (ALIX; PDCD6IP) and tumor susceptibility gene 101 (TSG101) were higher in folic acid-injected rats than in control rats. LC-MS/MS analysis of urinary exosomal proteins from the folic acid-injected and control rats identified 372 proteins, including various potential TI fibrosis biomarkers Conclusion: We demonstrated the feasibility of using a folic acid-induced rat model of TI fibrosis for urinary proteomic analysis. These rats excreted sufficient urinary exosomes permitting the large-scale analysis and discovery of potential biomarkers of renal fibrosis	en
dc.language.iso	en_US	en
dc.subject	Biomarkers	en
dc.subject	ตัวบ่งชี้ทางชีวภาพ	en
dc.subject	Kidneys -- Diseases	en
dc.subject	ไต – โรค	en
dc.subject	Chronic kidney disease	en
dc.subject	โรคไตเรื้อรัง	en
dc.subject	Folic acid	en
dc.subject	กรดโฟลิก	en
dc.subject	Interstitial fibrosis	en
dc.subject	อินเทอร์สทิเซียลไฟโบรซิส	en
dc.subject	เนื้อเยื่อพังผืดระหว่างเซลล์กล้ามเนื้อ	en
dc.subject	Proteomics	en
dc.subject	โปรตีโอมิกส์	en
dc.subject	Urinary exosomes	en
dc.title	A folic acid-induced rat model of renal injury to identify biomarkers of tubulointerstitial fibrosis from urinary exosomes	en
dc.type	Article	en