Screening and Producing of Polyhydroxyalkanoated (PHAs) from Soil Bacteria

Abstract

PHAs granular accumulating bacteria cell isolated from Donmuang landfill soil, Bangkok, Thailand, three of isolates, DM12-22, DM16-11 and DM30-11 shown high intracellular PHAs accumulation when cultured in mineral salt medium supplemented with molasses as carbon source and then screened by using of Sudan Back B straining technique. After pure culture in mineral salt broth fermentor, growth rate and PHAs accumulation were investigated. For growth rate, DM12-22 showed highest growth rate within 48 hr of culturing period. In addition, for PHAs accumulation, DM30-11 showed highest PHAs compared with DM-16-11 and DM12-22, were 0.092 g/l-1 (0.49% of dry weight), 0.065 g/l-1 (0.41% of dry weight), and 0.037 g/l-1 (0.18% of dry weight), respectively.