Optimization of Lipase Production from Lipase-Producing Bacteria in Soil Using Waster of Industrial Vegetable Oil Production

Abstract

Lipase production by the isolate HCU07 was optimized by varying environmental and nutritional conditions in culture medium containing different used vegetable oils and the waste of industrial vegetable oil production at a concentration of 2% (v/v) of basal medium with shaking at 200 rpm for 24 h at room temperature (27-30 °C). The maximum lipase production was obtained using palm fatty acid distillates from oil refining process (16.57+-0.62 U/mg), followed in order by used soybean oil (16.16+-0.77 U/mg), used palm oil (14.29+-0.52 U/mg) and washed water from oil refining process (12.99+-0.22 U/mg), respectively. The highest lipase activity (27.57+-0.33 U/mg) was obtained when soytone was used at concentration of 0.5% (v/v) of basal medium. The optimal pH and temperature for lipase production were pH 7 (28.03+-0.12 U/mg) and room temperature (27-30 °C) (28.53+-0.12C U/mg), respectively. Zymogram assay of crude enzyme on SDS-PAGE presented one bands with lipase activity of molecular weight of 21.5 kDa. The enzyme has good potential for the hydrolysis of used vegetable oils and the waste of industrial vegetable oil production, which are industrially and economically important for industrial applications.