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Title: | Antibacterial efficacy of Citrus hystrix (makrut lime) essential oil against clinical multidrug-resistant methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates |
Authors: | Preeyaporn M. Sreepian Panthip Rattanasinganchan Apichai Sreepian Rangsit University. Faculty of Medical Technology Huachiew Chalermprakiet University. Faculty of Medical Technology Rangsit University. Faculty of Medical Technology |
Keywords: | ฤทธิ์ต้านเชื้อแบคทีเรีย Antibacterial activity พิษต่อเซลล์ Cytotoxicity มะกรูด Citrus hystrix การดื้อยาหลายชนิด Multidrug resistance Methicillin-Resistant Staphylococcus Aureus |
Issue Date: | 2023 |
Citation: | Saudi Pharmaceutical Journal 31, 6, (June 2023) : 1094-1103 |
Abstract: | The increasing incidence of methicillin-resistant S. aureus is a major public health concern. Recently, the performance of Citrus hystrix essential oil (CHEO) has been shown to contain broad-spectrum antibacterial activity. Therefore, this study aims to determine the antibacterial activity of CHEO alone and in combination with gentamicin against panels of clinical isolates of methicillin-susceptible S. aureus (MSSA, n = 45) and methicillin-resistant S. aureus (MRSA, n = 40). Antibiotic susceptibility testing revealed multidrug-resistant (MDR) patterns among 3 MSSA isolates and 39 MRSA isolates, indicating that the clinical MRSA isolates were associated with MDR (p < 0.05). For the drug resistant isolates, resistance was observed toward most antibiotics, except for chloramphenicol, trimethoprim-sulfamethoxazole, linezolid, and vancomycin. Antibacterial screening by disk diffusion demonstrated that CHEO alone had certain antibacterial activity toward all MSSA isolates (IZD: 16.0 ± 4.7 mm) and MRSA isolates (IZD: 16.5 ± 4.2 mm) (p > 0.05). The MIC values of CHEO are 18.3 ± 6.1 mg/mL in MSSA isolates and 17.9 ± 6.9 mg/mL in MRSA isolates (p > 0.05). The antibacterial activity of CHEO demonstrated the bactericidal effect with MIC index 1.0-1.4. Time-killing kinetics revealed that CHEO at 1 × MIC completely killed MSSA and MRSA within 12 h. Moreover, the checkerboard titration demonstrated the synergistic and additive interactions of CHEO with gentamicin with FIC index 0.012-0.625. CHEO against human epidermal keratinocyte; HaCaT cell line demonstrated the IC50 value at 2.15 mg/mL. The use of CHEO as an alternative antibacterial agent would reduce the emergence of resistant bacteria, especially MDR MRSA. |
Description: | สามารถเข้าถึงบทความฉบับเต็มได้ที่ https://doi.org/10.1016/j.jsps.2023.03.020 |
URI: | https://has.hcu.ac.th/jspui/handle/123456789/2053 |
Appears in Collections: | Medical Technology - Artical Journals |
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File | Description | Size | Format | |
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Citrus-hystrix .pdf | 96.25 kB | Adobe PDF | View/Open |
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