Simultaneous Determination of Diosmin and Hesperidin in Cissus quadrangularis Capsules by Ultraviolet-Visible Spectroscopy

Abstract

The aim of this study was to develop and validate the analytical method for simultaneous determination of diosmin and hesperidin in Cissus quadrangularis capsules, using UV-visible spectroscopy based on simultaneous equation method. Standard and sample solutions were prepared in 0.2 N NaOH and measured the absorbances at 267.5 and 284.0 nm, as the maximum absorption wavelength of diosmin and hesperidin, respectively. Validation results of showed acceptable linearity of diosmin and hesperidin over the concentration range of 1 – 5 and 4.8 – 24 μg/mL, respectively. Unfortunately, the percentage recoveries and %RSD for hesperidin did not meet the acceptance criteria. The developed method was applied to quantify the content of diosmin and hesperidin in the capsules, the assay result revealed that each 250-mg capsule (125 mg of C. quadrangularis powder) contained 29.88 ± 0.08 and 3.176 ± 0.45 mg of diosmin and hesperidin, respectively. However, more specific and precise method, such as high-performance liquid chromatography (HPLC) must be further developed for the estimation of diosmin and hesperidin in multicomponent formulations.