Gelatin was extracted from scale of Snakeskin gourami (Trichogaster pectoralis) fish by low alkaline extraction process. The aim of the study was to determine the optimal conditions for preparing gelatin using different sodium hydroxide concentrations. Results showed that the fish gelatin were slight yellow-white colour, translucent, light textured in appearance and least of fishy odour. The yield of gelatin extraction from 0.3% sodium hydroxide as possessed for 1 and 3 hours soaking time were 9.86% and 9.44%, respectively. The highest viscosity value 368 RVU of gelatin was obtained from fish scale by use 0.3% sodium hydroxide soaking for 3 hours. However, in preliminary treatment that used 0.1% sodium hydroxide solution, clearly liquid gel with higher setting temperature at 23 °C and minimum setting time of 13 seconds was obtained.